Acta Ichthyologica et Piscatoria 12(2): 41-49, doi: 10.3750/AIP1982.12.2.04
Properties of tench - Tinca tinca L. sperm and experiments with freezing it at -196°C
expand article infoM. Moczarski, M. Kołdras
Open Access
Properties of tench sperm were studied by means of the following indices: sperm volume, spermatozoa concentration, total number of spermatozoa, spermatocrit, and sperm motility. A series of diluting media was tested in order to find optimal conditions for tench sperm freezing; phosphate buffer (BF) enriched by a 15% addition of ethylene glycol (EG) was found to be the most suitable diluting medium.