Corresponding author: Yazdan Keivany ( keivany@iut.ac.ir ) Academic editor: Mirosław Przybylski
© 2021 Neda Dastanpoor, Yazdan Keivany, Eisa Ebrahimi.
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Citation:
Dastanpoor N, Keivany Y, Ebrahimi E (2021) Comparative osteology of three endemic cichlids (Iranocichla spp.) (Actinopterygii, Perciformes, Cichlidae) from southern Iran. Acta Ichthyologica et Piscatoria 51(1): 67-75. https://doi.org/10.3897/aiep.51.63218
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Iranian cichlids are isolated disjunct populations of the African cichlids group, restricted to the Hormuz Strait region in the Hormuz basin, and are a very important group from both zoogeographic and phylogenetic points of view. Thus, the osteological structures, as one of the most reliable structures, of the three nominal species of Iranian cichlid fishes, Iranocichla hormuzensis Coad, 1982, from the Mehran River, Iranocichla persa Esmaeili, Sayyadzadeh et Seehausen, 2016, from Khorgo hot spring, and Iranocichla sp., from the Kol River were described and compared. Ten specimens of each species were cleared and stained with Alcian blue and Alizarin red. A digital camera was used for taking pictures and CorelDraw X6 software for preparing drawings. The three species have some differences in the caudal fin skeleton, shape of the urostyle, neural spine of preural 2, hypural spines 2, and 3, anterior part of parhypural and hypurapophysis, the size of neural spines of preural 2 and 3, hypural 3 and 4, and epurals. Although there are some differences among the species, osteology of these species is very conservative and other traits like behavioral and molecular should be used.
Biodiversity, bone, Cichlidae, Iranocichla, osteology
The family Cichlidae contains about 202 genera and more than 2000 species. The geographical distribution of freshwater cichlids includes Jordan Valley, southern Iran, Sri Lanka and southern India, Cuba and Hispaniola, Madagascar, Africa, central, southern, and North America (
Thirty specimens of Iranocichla spp. with 3–5.5 cm total length collected from Mehran and Kol rivers and Khorgo hot spring in southern Iran, were examined. They were kept in ethanol (70%) and stored at Isfahan University of Technology Ichthyology Museum (IUT-IM). They were cleared and stained with Alizarin red and Alcian blue according to
Cranium (Fig.
Dorsal (a) and ventral (b) views of the cranium in Iranocichla hormuzensis. Boc: Basioccipital; Epo: Epiotic; Eth: Ethmoid, attached to the surroundings by cartilage not shown in the figure; Exo: Exoccipital; Fr: Frontal; F.exo: foramen exoccipital; Le: Lateral ethmoid; Nas: Nasal; Os: Orbitosphenoid; Pa: Parietal; Pf: Prootic foramen; Pro: Prootic; Ps: Parasphenoid; Ptr: Pterotic; Pvo: Prevomer; Sph: Sphenoid; Soc: Supraoccipital; So-cr: Supraoccipital crest.
The orbital region consists of the paired orbitosphenoids, circumorbital series, frontals and the unpaired parasphenoid. The frontal is a large element of skull roof with an elongated posterior edge which is linked to the sphenotic and parietal (Fig.
The parietals are situated between the supraoccipital and frontal which are linked to each other in the midline. Epiotic is located between parietal, supraoccipital, exoccipital, and pterotic. This bone has an enlarged process in the posteriodorsal edge. The pterotic is nearly triangular which is ventrally connected to the parietal and epiotic, and laterally to the prootic. The prootic ventrally contacts the orbitosphenoid and frontal, dorsally the sphenotic and posteriorly the basioccipital and pterotic. There is a foramen in the prootic for the passage of auditory nerves and blood vessels. The sphenotic bears a small process anterodorsally. The supraoccipital is wide in the middle and linked to the parietal by its anterior process and posteriolaterally to the exoccipitals and epiotic and bears a blade-shaped crest. The exoccipital is linked to the pterotic laterally and bears a foramen on its ventral part. The basioccipital is almost jug-shaped that is cheeky in its middle portion. This bone is located between the prootic and exoccipitals in the lateral view of the skull (Fig.
Jaws (Figs
The suspensorium and opercular series and upper jaw in Iranocichla hormuzensis. Abbreviations: An: Angular; Dn: Dentary; Ect: Ectopterygoid; End: Endopterygoid; Hy: Hyomandibular; Inp: Interopercle; Mc: Meckel cartilage Mtp: Metapterygoid; Op: Opercle; P: Palatine; Pop: Preopercle; Q: Quadrate; Ra: Retroarticular; Sop: Subopercle; Sym: Symplectic.
Suspensorium (Fig.
Opercular Series (Fig.
Hyoid arch (Fig.
(a) Hyoid arch of Iranocichla hormuzensis. Bhy: Basihyal, Brs 1–5: Branchiostegal 1–5; Chy: Ceratohyal, Dhy and Vhy: Dorsal and Ventral hypohyal; Ehy: Epihyal; Ihy: Interhyal; Uhy: Urohyal. (b) Branchial apparatus of I. hormuzensis. Bbr: Basibranchial; Cbr: Ceratobranchial (Cbr5: Pharyngeal bones); Ebr: Epibranchial: Hbr: Hypobranchial; Pbr: Pharyngobranchials.
Branchial arch (Fig.
Vertebral columns (Fig.
Lateral view of vertebrae (a), dorsal fin (b) and anal fin (c) of Iranocichla hormuzensis. Abbreviations: Afs: Anal fin spine; C3–C4, C15–C21: Centrum 3–4, Centrum 15–21; Dfs: Dorsal fin spine; Dpt: Distal part of the pterygiophore; Hec: Hemal canal; Hsp (1): Hemal spine (1); Na: Neural arch; Ns: Neural spine; Pd: Predorsal; Pt: Pterygiophore; R: Rib; Sty: Stay; Zyg: Zygopophysis.
Dorsal and anal fins (Fig.
Lateral view of the caudal skeletons of Iranocichla hormuzensis (a), Iranocichla sp. (b) and I. persa (c). Abbreviations: Epu 1–2: Epural 1–2; Hp 1–5: Hypurals 1–5; Hs2: Haemal spine 2; hpp: Hypurapophysis; Ns: Neural spine; Phy: Parhypural; Pu2 (3): Preurals 2 (4); Urn: Uroneural; Urs: Urostyle.
Caudal skeleton (Fig.
Comparison of the caudal skeletons of Iranocichla hormuzensis, Iranocichla persa and Iranocichla sp.
PHY, URN, URS, HS2 | HS3 | NS3 | NS2 | Epu1-2 | Hyp5 | Hyp1-4 | Character / Species | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. | Length | No. | Length | No. | Status | No. | Status | No. | Status | |||||||
3,4 | 1,2 | |||||||||||||||
Slight difference in shape | 2 elements (first: elongated second: short) | 6 | Elongated | 1 | Both elongated | 2 elements | 2 | Both free, second longer | 2 elements | 2 | Wider posteriorly | All: free (wedge-shaped) | 5 | No fusion | No fusion | I. hormuzensis |
1 | First: free second: fused with pu2 | 1 | Complete fusion | Complete fusion | ||||||||||||
7 | Narrow anteriorly | |||||||||||||||
2 | First: short, second: elongated | |||||||||||||||
2 | Attached to each other with curvature | |||||||||||||||
2 | Attached to each other without curvature | 1 | Complete fusion | No fusion | ||||||||||||
1 element: elongated | 4 | Short | 1 | First: short, second: elongated (fused together) | ||||||||||||
1 | Fused to each other | 1 | Anterior needle-shaped | 1 | No fusion | Incomplete fusion | ||||||||||
5 | Short | 1 element | 1 | Without curvature | 1 element | |||||||||||
1 | Incomplete fusion | No fusion | ||||||||||||||
1 | Short (fused with epu2) | 1 | With curvature | |||||||||||||
1 | Incomplete fusion | Incomplete fusion | ||||||||||||||
Slight difference in shape | 1 element: elongated | 6 | Elongated | 1 | both Short | 2 elements | 6 | Both free, second longer | 2 elements | 9 | All free (wedge-shaped of different sizes) | 5 | No fusion | No fusion | Iranocichla sp. | |
1 | Complete fusion | No fusion | ||||||||||||||
3 | First: short second: elongated | |||||||||||||||
4 | Attached to each other | 3 | No fusion | Incomplete fusion | ||||||||||||
1 element: short* | 4 | Short | ||||||||||||||
6 | Short | 1 element | 1 | Bar-shaped | 1 | Incomplete fusion | Incomplete fusion | |||||||||
Slight difference in shape | 1 element: elongated | 7 | Elongated | 3 | First: short, second: elongated | 2 elements | 5 | Both free, second longer with flat end | 2 elements | 10 | All free (wedge-shaped of different sizes) | 3 | No fusion | No fusion | I. persa | |
2 | Complete fusion | Complete fusion | ||||||||||||||
1 | Complete fusion | No fusion | ||||||||||||||
5 | Both free, second longer with hook-shaped end |
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1 element: branched | 3 | Short | ||||||||||||||
1 | No fusion | Complete fusion | ||||||||||||||
7 | Short | 1 element | ||||||||||||||
3 | No fusion | Incomplete fusion |
Pectoral girdle (Fig.
Lateral view of the pectoral girdle (a) and internal view of the pelvic (b) of Iranocichla hormuzensis. Abbreviations: Act: Actinost; Anp: Anteroventral Process; Bpt: Basipterygium; Cl: Cleithrum; Cor; Coracoid; Dp: Distal process; Pcl 1, 2; Postcleithrum 1, 2; Pfr: Pectoral fin ray; Pfs: Pelvic fin spine; Pop: Posterior process; Ptt: Posttemporal; Sc: Scapula; Scl: Supracleithrum.
Pelvic girdle (Fig.
The osteology of three endemic species of Iranian cichlid, Iranocichla hormuzensis, I. persa, and Iranocichla sp. is described and compared for the first time.
The majority of the bony elements including the neurocranium and the branchiocranium were basically similar in all the three species and did not show any significant differences among the species. The mesethmoid is found in many species of tilapia but the genera Sarotherodon, Oreochromis, and Iranocichla are reported to lack this bone (
The presence of two types of oral and pharyngeal teeth and the parental care system in Cichlids has increased various feeding strategies and increased the survival of larvae and population (
We would like to thank E. Daneshvar, for collecting the fishes, and M. Zamani-Faradonbe for his assistance in laboratory work. This study was financially supported by the Isfahan University of Technology.